SARS-CoV-2/influenza A/influenza B
Produkt Namme
HWTS-RT148-SARS-CoV-2/influenza A/influenza B Nucleic Acid Combined Detection Kit (Fluorescence PCR)
Kanaal
| Kanaal Namme | PCR-Mix 1 | PCR-Mix 2 |
| FAM Channel | ORF1ab gen | IVA |
| VIC / HEX Channel | Ynterne kontrôle | Ynterne kontrôle |
| CY5 kanaal | N gen | / |
| ROX Channel | E gene | IVB |
Technyske parameters
| Opslach | -18 ℃ |
| Shelf-life | 12 moanne |
| Soart eksimplaar | nasopharyngeale swabs en orofaryngeale swabs |
| Doel | SARS-CoV-2 trije doelen (Orf1ab, N en E genen) / gryp A / gryp B |
| Ct | ≤38 |
| CV | ≤10,0% |
| LoD | SARS-CoV-2: 300 kopyen/ml influenza A firus: 500 kopyen/ml influenza B firus: 500 kopyen/ml |
| Spesifisiteit | a) De resultaten fan 'e krústest lieten sjen dat de kit kompatibel wie mei minsklik coronavirus SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respiratory syncytial firus A en B, parainfluenza firus 1, 2 en 3, rhinovirus A, B en C, adenovirus 1, 2, 3, 4, 5, 7 en 55, minsklik metapneumovirus, enterovirus A, B, C en D, minsklik cytoplasmysk pulmonary firus, EB firus, masels firus Human cytomegalovirus, rotavirus, norovirus, bof firus, varicella zoster firus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Mycoplasma pneumoniae, Mycoplasma pneumoniae, Legionella, pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Mycoplasma pneumoniae, Klebsiella pneumeiga, Klebsiella pneumeiga, Asillutus influenzae, s, Candida glabrata Der wie gjin krúsreaksje tusken Pneumocystis yersini en Cryptococcus neoformans. b) Anti-ynterferinsjefermogen: selektearje mucin (60mg/mL), 10% (V/V) minsklik bloed, diphenylephrine (2mg/mL), hydroxymethylzoline (2mg/mL), natriumchloride (befettet konserveringsmiddel) (20mg/mL), beclomethason (20mg/mL), dexamethason (20mg/mL), flunison (20μg/mL), triamcinoloneacetonide (2mg/mL), budesonide (2mg/mL), mometason (2mg/mL), fluticason (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL). ), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceprotene (40μg/mL) Meropenem (200mg/mL), levofloxacin (10μg/mL) en tobramycin (0.6mg/mL) .De resultaten lieten sjen dat de interferearjende stoffen by de boppesteande konsintraasjes gjin ynterferinsjereaksje hienen op de deteksjeresultaten fan sykteferwekkers. |
| Tapasbere ynstruminten | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Real-Time PCR-systemen LightCycler®480 Real-Time PCR systeem LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Totale PCR-oplossing
