SARS-CoV-2/gryp A/gryp B
Produktnamme
HWTS-RT148-SARS-CoV-2/gryp A/gryp B Kombineare Nukleïnezuurdeteksjekit (Fluoreszinsje-PCR)
Kanaal
| Kanaalnamme | PCR-Mix 1 | PCR-Mix 2 |
| FAM-kanaal | ORF1ab-gen | IVA |
| VIC/HEX-kanaal | Ynterne kontrôle | Ynterne kontrôle |
| CY5-kanaal | N-gen | / |
| ROX-kanaal | E-gen | IVB |
Technyske parameters
| Opslach | -18℃ |
| Hâldberens | 12 moannen |
| Spesimentype | nasofaryngeale swabs en orofaryngeale swabs |
| Doel | SARS-CoV-2 trije doelen (Orf1ab-, N- en E-genen) / gryp A / gryp B |
| Ct | ≤38 |
| CV | ≤10,0% |
| LoD | SARS-CoV-2: 300 eksimplaren/ml grypfirus A: 500 eksimplaren/ml grypfirus B: 500 eksimplaren/ml |
| Spesifisiteit | a) De krústestresultaten lieten sjen dat de kit kompatibel wie mei it minsklike coronavirus SARSr-CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, respiratoir syncytial firus A en B, parainfluenza firus 1, 2 en 3, rhinovirus A, B en C, adenovirus 1, 2, 3, 4, 5, 7 en 55, minsklik metapneumovirus, enterovirus A, B, C en D, minsklik cytoplasmatysk longfirus, EB-firus, mazelenfirus, minsklik cytomegalovirus, rotavirus, norovirus, boffirus, varicella zosterfirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella, kinkhoest, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata Der wie gjin krúsreaksje tusken Pneumocystis yersini en Cryptococcus neoformans. b) Anti-ynterferinsjefermogen: selektearre mucine (60mg/mL), 10% (V/V) minsklik bloed, difenylefrine (2mg/mL), hydroxymethylzoline (2mg/mL), natriumchloride (mei conserveringsmiddel) (20mg/mL), beclomethason (20mg/mL), dexamethason (20mg/mL), flunison (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometason (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), α-Interferon (800IU/mL), zanamivir (20mg/mL), ribavirine (10mg/mL), oseltamivir (60ng/mL), pramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocine (20mg/mL), azitromycine (1mg/mL), ceproteen (40μg/mL) Meropenem (200mg/mL), levofloxacine (10μg/mL) en tobramycine (0.6mg/mL). De resultaten lieten sjen dat de ynterferearjende stoffen by de boppesteande konsintraasjes gjin ynterferinsjereaksje hiene op de deteksjeresultaten fan patogenen. |
| Tapasselike ynstruminten | Applied Biosystems 7500 Real-Time PCR Systemen Applied Biosystems 7500 Snelle Real-Time PCR-systemen SLAN ®-96P Real-Time PCR-systemen QuantStudio™ 5 Real-Time PCR-systemen Ljochtsykler®480 Real-Time PCR-systeem LineGene 9600 Plus Real-Time PCR-deteksjesysteem MA-6000 Real-Time Kwantitative Termyske Sykler BioRad CFX96 Real-Time PCR-systeem BioRad CFX Opus 96 Real-Time PCR-systeem |
Totale PCR-oplossing
