Respiratory pathogens kombinearre
Produkt Namme
HWTS-RT050-Seis soarten Respiratory Pathogen Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Influenza, yn 't algemien bekend as 'gryp', is in akute respiratoaryske ynfeksjesykte dy't feroarsake wurdt troch influenzafirus, dat tige besmetlik is en benammen oerbrocht wurdt troch hoesten en niezen.
Respiratory syncytial firus (RSV) is in RNA-firus, dat heart ta de famylje paramyxoviridae.
Human adenovirus (HAdV) is in dûbelstrenged DNA-firus sûnder envelope.Op syn minst 90 genotypen binne fûn, dy't kinne wurde ferdield yn 7 subgenera AG.
Human rhinovirus (HRV) is in lid fan 'e Picornaviridae famylje en de Enterovirus genus.
Mycoplasma pneumoniae (MP) is in patogene mikroorganisme dat tusken baktearjes en firussen yn grutte is.
Kanaal
| Kanaal | PCR-Mix A | PCR-Mix B |
| FAM Channel | IFV A | HAdV |
| VIC / HEX Channel | HRV | IFV B |
| CY5 kanaal | RSV | MP |
| ROX Channel | Ynterne kontrôle | Ynterne kontrôle |
Technyske parameters
| Opslach | -18 ℃ |
| Shelf-life | 12 moanne |
| Soart eksimplaar | Orofaryngeal swab |
| Ct | ≤35 |
| LoD | 500 kopyen/ml |
| Spesifisiteit | 1.De resultaten fan krúsreaktiviteitstests lieten sjen dat d'r gjin krusreaksje wie tusken de kit en minsklik coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, Parainfluenza firus typen 1, 2, en 3, Chlamydia pneumoniae, minsklik metapneumovirus, Enterovirus A, B, C, D, Epstein-Barr firus, Measles firus, minsklik cytomegalovirus, Rotavirus, Norovirus, Mumps firus, Varicella-zoster firus, Legionella, Bordetella pertussis, Haemophilus influlocenzae, aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans en minsklike genomyske nucleic soeren. 2.Anti-ynterferinsjefermogen: Mucin (60mg/mL), 10% (v/v) minsklik bloed, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), natriumchloride (mei conserveringsmiddelen) (20mg/mL), beclomethasone ( 20mg/mL), dexamethason (20mg/mL), flunisolide (20μg/mL), triamcinoloneacetonide (2mg/mL), budesonide (2mg/mL), mometason (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alfa-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), en tobramycin (0.6mg/mL) waarden selektearre foar de ynterferinsjetest, en de resultaten lieten sjen dat de interferearjende stoffen by de boppesteande konsintraasjes gjin ynterferinsjereaksje hienen op de testresultaten fan patogenen. |
| Tapasbere ynstruminten | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR systeem LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR-systeem, BioRad CFX Opus 96 Real-Time PCR-systeem |
Totale PCR-oplossing
